(Tungstophosphoric Acid)
H3[P(W3O10)4] aq F.W. 2880.17+aq CAS #12501-23-4
(Tungstophosphoric Acid)
H3[P(W3O10)4] aq F.W. 2880.17+aq CAS #12501-23-4
Specifications:
|
Appearance |
|
Practically clear, |
|
Ammonia (NH ) |
|
0.004% |
|
Chloride (Cl) |
|
0.03% |
|
Insoluble Matter |
|
0.02% |
|
Nitrate (NO3 ) |
|
0.004% |
|
Sulfate (SO4 ) |
|
0.005% |
|
Heavy Metals and Iron. |
|
No color formation on |
Used as a Fixative:
Issidorides, M.R., and Kasorchis, T. (1981).
Dispersed and compact chromatin demonstrated with a new EM Method: phosphotungstic acid-hematoxylin block-staining. Histochemistry 73,21.
Used widely as a Negative Stain:
As Spray method: Horne, R.W., and Pasquali-Ronchette, I. (1974). A negative staining-carbon film technique for studying viruses in the electron microscope.
Preparation procedures for examining icosahedral and filamentous viruses. J. Ultrastruct. Res. 47, 361. Locke, M., and Kirhman, N. (1971).
Hot Alcoholic phosphotungstic acid and uranyl acetate as routine stains for thick and thin sections. J. Cell Biol., 50, 550.,Farragiana, T., and Marinozzi, V. (1979).
Phosphotungstic acid staining of polysaccharides containing structures on epoxy embedded tissues. J. Submicrosc. Cytol. 11, 263.Bloom, E.E., And Aghajanian, G.K. (1968).
Fine structural and cytochemical analysis of the staining of synaptic junctions with PTA. J. Ultrastruct. Res. 22, 261.
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